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探讨应用Han等人方法建立新生大鼠脑白质损害(White Matter Damage,WMD)模型的远期可行性。方法选2日龄新生SD大鼠32只,随机分为正常对照组、假手术组及实验组。实验组大鼠在乙醚麻醉下行右颈总动脉结扎木,术后吸6％氮氧混合气4h,制作新生大鼠WMD模型。正常饲养至1月龄时检测感觉运动功能及情感行为能力。之后经灌注固定行常规HE染色及电镜检查。结果实验组大鼠感觉运动功能及情感行为能力较正常对照组明显减退。悬吊试验实验组得分1.6±0.7,假手术组得分3.1±0.7,正常对照组得分3.4±1.00;斜坡试验得分分别为7.1±2.4、3.3±1.1、3.2±1.4;旷场试验得分分别为4.6±2.7、12.3±2.8、12.0±3.8。HE染色可见实验组患侧白质区有不同程度纤维疏松。电镜可见实验组患侧海马CAI区胶质细胞受损及髓鞘形成延迟。假手术组及正常对照组均无此表现。结论该方法对于研究早产儿WMD简单可靠。
To investigate the long-term feasibility of establishing the model of white matter damage (WMD) in neonatal rats by the method of Han et al. Methods Thirty-two SD neonatal SD rats were randomly divided into normal control group, sham operation group and experimental group. In the experimental group, the right common carotid artery ligation was performed under ether anesthesia, and the rats were pretreated with 6% oxynitride for 4 hours to make WMD model of neonatal rats. Normal feeding to 1 month of age to detect sensory and motor function and emotional behavior. After perfusion fixation routine HE staining and electron microscopy. Results The sensory function and emotional behavior of experimental group rats were significantly lower than those of normal control group. The scores of sham test group were 1.6 ± 0.7, those of sham group were 3.1 ± 0.7, those of control group were 3.4 ± 1.00; those of slope group were 7.1 ± 2.4, 3.3 ± 1.1 and 3.2 ± 1.4 respectively; the scores of open field test were 4.6 ± 2.7, 12.3 ± 2.8, 12.0 ± 3.8. Hematoxylin-eosin staining showed that there were varying degrees of fibrosis in the white matter area of the experimental group. Electron microscopy showed that the injured hippocampal CAI region of the experimental group glial cells and delayed myelination. Sham-operated group and normal control group were no such performance. Conclusion This method is simple and reliable for studying WMD in preterm infants.