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目的:探讨三七总皂苷对脑缺血再灌注损伤的保护作用及机制。方法:SD大鼠分成假手术组,模型组,三七总皂苷组。模型组和三七总皂苷组采用线栓法建立大鼠大脑中动脉局灶缺血再灌注损伤模型,三七总皂苷组在缺血前15 min和缺血后6 h分别ip三七总皂苷100 mg.g-1,模型组ip等量生理盐水。术后24 h评价神经行为学变化,测定脑梗死体积,脑组织中伊文思蓝含量及白介素-β(IL-1β),肿瘤坏死因子α(TNF-α),白介素-6(IL-6),白介素-8(IL-8)水平。结果:三七总皂苷可有效降低脑缺血再灌注损伤大鼠的脑梗死体积,明显减轻脑缺血区血脑屏障破坏的程度。三七总皂苷组脑组织中细胞因子及伊文思蓝的含量较缺血再灌注组显著降低(P<0.05)。结论:三七总皂苷对脑缺血再灌注损伤有显著的脑保护作用,其作用机制与降低脑缺血再灌注损伤中脑部细胞因子的表达和降低血脑屏障通透性有关。
Objective: To investigate the protective effect of Panax Notoginseng Saponins on cerebral ischemia-reperfusion injury and its mechanism. Methods: SD rats were divided into sham operation group, model group and Panax notoginseng saponin group. The model group and the Panax notoginseng saponin group were established by focal occlusion of middle cerebral artery focal ischemia-reperfusion injury model, Panax notoginseng saponins group 15 min before ischemia and 6 h after ischemia were notoginseng total saponins 100 mg.g-1, the model group ip equivalent saline. Neurobehavioral changes were evaluated at 24 hours after operation. The volume of cerebral infarction and the content of Evans blue and the levels of interleukin-1β, tumor necrosis factor-α (TNF-α), interleukin-6 (IL- , Interleukin-8 (IL-8) levels. Results: Panax Notoginseng Saponins could effectively reduce the volume of cerebral infarction in rats with cerebral ischemia-reperfusion injury and significantly alleviate the damage of blood-brain barrier in cerebral ischemia. The contents of cytokines and Evans blue in the brain of Panax notoginseng saponins group were significantly lower than those of ischemia-reperfusion group (P <0.05). CONCLUSION: Panax notoginseng has a significant cerebral protective effect on cerebral ischemia-reperfusion injury. Its mechanism may be related to reducing the expression of cytokines in the brain and decreasing the permeability of the blood-brain barrier during cerebral ischemia-reperfusion injury.