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目的 :探讨p53、PCNA、VEGF表达与食管鳞状细胞癌临床病理特性及预后之间的关系。方法 :用免疫组织化学SABC法 ,测定 10 0份食管癌组织的p53、PCNA及VEGF表达 ,分析其与临床病理因素及预后的关系 ,结果 :p53在食管鳞癌中的阳性表达与肿瘤浸润深度有关 ;PCNA阳性表达与肿瘤细胞分化程度、浸润深度、淋巴结转移状况有关 ;VEGF阳性表达与肿瘤细胞分化程度、肿瘤浸润深度相关 ;VEGF与生存期呈负相关。 3项基因表达阳性患者生存期明显短于单项表达阳性或 3项均阴性患者。结论 :食管鳞状细胞癌组织中p53、PCNA及VEGF表达阳性反映肿瘤的生物学行为。VEGF与食管鳞癌的恶性进程和不良预后有密切关系 ,是食管鳞癌一个独立预后因素。
Objective: To investigate the relationship between the expression of p53, PCNA and VEGF and the clinicopathological characteristics and prognosis of esophageal squamous cell carcinoma. Methods: Immunohistochemical SABC method was used to determine the expression of p53, PCNA and VEGF in 10 esophageal cancer tissues. The relationship between the expression of p53 and clinicopathological parameters and prognosis was analyzed. Results: The positive expression of p53 in esophageal squamous cell carcinoma was correlated with the depth of tumor invasion The positive expression of PCNA correlated with the degree of tumor differentiation, depth of invasion and lymph node metastasis. The positive expression of VEGF was correlated with the degree of tumor cell differentiation and the depth of tumor invasion. There was a negative correlation between the expression of VEGF and the survival time. The survival of 3 gene positive patients was significantly shorter than that of single positive or 3 negative patients. Conclusion: The positive expression of p53, PCNA and VEGF in esophageal squamous cell carcinoma reflect the biological behavior of tumor. VEGF and esophageal squamous cell carcinoma is closely related to the malignant process and poor prognosis, esophageal squamous cell carcinoma is an independent prognostic factor.