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用人体巨噬细胞体外加SiO_2培养,提纯抗原,免疫BALB/C小鼠,获取致敏的小鼠脾细胞与SP_2/O骨髓瘤细胞融合,克隆化,筛选出两株分泌抗尘细胞单克隆抗体的杂交瘤细胞。用其分泌的单克隆抗体测定了79例各期矽肺病人,54例正常人及32例肺科病人血清,阳性率分别为94.9％、3.7％、6.2％。各期矽肺血清与单克隆抗体反应测定均值呈梯度关系有高度显著性差异。在可疑矽肺与正常人之间有明显的界限值。据此认为抗尘细胞单克隆抗体用于矽肺诊断及胸片前筛选具有一定的实用价值。
The human macrophages were cultured in vitro with SiO_2, antigen was purified and BALB / C mice were immunized. The sensitized mouse spleen cells were fused with SP 2 / O myeloma cells and cloned. Two McAbs secreting anti-dust cells Antibody hybridoma cells. The sera of 79 patients with stage silicosis, 54 normal subjects and 32 pulmonary patients were detected by their monoclonal antibodies. The positive rates were 94.9%, 3.7% and 6.2% respectively. There was a highly significant difference in the gradient between the mean of serum silicate and monoclonal antibody responses in each phase. There is a clear threshold between suspicious silicosis and normal people. Therefore, anti-dust cell monoclonal antibody for silicosis diagnosis and screening before screening has some practical value.