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目的探讨慢性乙型肝炎(慢乙肝)患者病毒复制水平与HBV标志物(HBV-M)模式及肝损害程度的关系。方法 360例慢性乙型肝炎(轻度160例、中度140例、重度60例)患者血清HBV DNA含量采用荧光标记(AmpliSensor)定量PCR方法检测,HBV-M检测采用ELISA法。结果血清HBV DNA含量与HBV-M模式有关,血清HBeAg阳性组HBV DNA含量(106.35±1.84)显著高于HBeAg阴性组(104.73±1.88)(P<0.01),慢乙肝轻度、中度患者HBV DNA含量[(105.58±1.92),(106.27±2.05)]与慢乙肝重度患者HBV DNA含量(105.73±1.90)相比较无显著性差异(P>0.05),且不同HBV DNA水平患者的TBil、ALT、AST差别无显著性(P>0.05)。结论血清HBeAg的存在影响HBV DNA的水平变化,随着肝损害程度的加重,慢性乙型肝炎轻度、中度、重度患者血清HBV DNA基因含量未发生显著变化,同时血清HBV DNA含量与TBil、ALT、AST水平无明显关系。
Objective To investigate the relationship between viral replication and HBV markers (HBV-M) and the degree of liver damage in patients with chronic hepatitis B (chronic hepatitis B). Methods Serum HBV DNA levels in 360 patients with chronic hepatitis B (mild 160, moderate 140, severe 60) were measured by AmpliSensor quantitative PCR and HBV-M by ELISA. Results Serum HBV DNA levels were correlated with HBV-M patterns. The serum HBeAg-positive HBV DNA levels (106.35 ± 1.84) were significantly higher than those in HBeAg-negative (104.73 ± 1.88) (P <0.01) There was no significant difference between the two groups (P> 0.05). The levels of DNA in TBil, ALT (105.58 ± 1.92), (106.27 ± 2.05) and HBV DNA levels in patients with severe chronic hepatitis B (105.73 ± 1.90) , AST no significant difference (P> 0.05). Conclusions The presence of serum HBeAg affects the level of HBV DNA. With the severity of liver damage, there is no significant change in serum HBV DNA level in mild, moderate and severe chronic hepatitis B patients, ALT, AST level no significant relationship.