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目的:采用D-半乳糖诱导的糖基化模型大鼠,研究葛根素(Radix Puerariae,RP)对蛋白糖基化反应的抑制作用。方法:D半-乳糖(150 mg/kg,ip)给药8周,诱导糖基化模型大鼠,并于第2周开始给予葛根素高(300 mg/kg)、中(150 mg/kg)、低(75 mg/kg)剂量处理6周。测定红细胞醛糖还原酶活性、糖化血红蛋白含量和血清果糖胺、晚期糖基化终末产物含量及胰岛素水平、SOD活性和MDA含量。结果:葛根素高、中剂量可明显降低D-半乳糖引起的模型大鼠血糖升高(P<0.01)及红细胞醛糖还原酶活性,并显著抑制糖基化产物的形成(P<0.01);同时,还可改善胰岛素敏感性,增加超氧化物歧化酶活性(P<0.01),而葛根素高剂量组可显著降低血清脂质过氧化物含量(P<0.01)。结论:葛根素对D-半乳糖诱导的大鼠糖基化反应具有明显的抑制作用。
OBJECTIVE: To investigate the inhibitory effect of Radix Puerariae (RP) on the glycosylation reaction in rat models with D-galactose-induced glycosylation. METHODS: D galactose (150 mg/kg, ip) was administered for 8 weeks, glycosylated model rats were induced, and puerarin high (300 mg/kg) and medium (150 mg/kg) were administered at the 2nd week. ), low (75 mg/kg) dose treatment for 6 weeks. The red blood cell aldose reductase activity, glycosylated hemoglobin content, serum fructosamine, advanced glycation end products and insulin levels, SOD activity, and MDA content were determined. RESULTS: High and middle doses of puerarin significantly decreased the blood glucose (P<0.01) and erythrocyte aldose reductase activity in model rats induced by D-galactose, and significantly inhibited the formation of glycosylation products (P<0.01). At the same time, it can also improve insulin sensitivity and increase the activity of superoxide dismutase (P<0.01), while the high-dose puerarin group can significantly reduce serum lipid peroxide content (P<0.01). Conclusion: Puerarin has a significant inhibitory effect on D-galactose-induced glycosylation in rats.