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目的:探讨吴茱萸汤对醋酸诱导的内脏痛模型小鼠的止痛作用。方法:在醋酸诱导的内脏痛模型小鼠上,结合扭体实验及免疫组织化学染色探查吴茱萸汤的止痛作用与TRPV1的关联。结果:与模型组对比,吴茱萸汤组可显著降低小鼠扭体行为差异极显著(P<0.01),同时,吴茱萸汤组小鼠第1次扭体行为出现时间明显延迟,差异显著(P<0.05);与正常组对比,模型组结肠组织中TRPV1阳性表达显著增多差异极显著(P<0.001),而吴茱萸汤组小鼠结肠粘膜层与肌层的TRPV1阳性表达明显减少,与模型组对比差异极显著(P<0.001)。结论:热敏通道TRPV1是吴茱萸汤发挥止痛作用的关键环节。
Objective: To investigate the analgesic effect of Evodia Decoction on acetic acid-induced visceral pain model mice. Methods: The acetic acid-induced visceral pain model mice, combined with writhing test and immunohistochemistry to explore Evodia Decoction analgesic effect and TRPV1 association. Results: Compared with the model group, the Evodia Decoction group significantly reduced the writhing behavior of the mice (P <0.01). At the same time, the first writhing behavior of the Evodia Decoction group was significantly delayed (P < 0.05). Compared with the normal group, the TRPV1 positive expression in the model group significantly increased (P <0.001), but the TRPV1 positive expression in the mucosal layer and muscular layer of the Evodia Decoction group was significantly decreased, compared with the model group The difference was significant (P <0.001). Conclusion: TRPV1, a thermosensitive channel, is the key point of analgesic effect of Evodia Decoction.