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线粒体外膜蛋白电压依赖性阴离子通道(VDAC)是动物细胞凋亡调控系统中的关键组分,但其在植物中的功能还不明确。拟南芥VDAC家族有4个成员,它们都能被病原菌诱导,VDAC1在由病原菌所引起的超敏性细胞死亡中发挥作用已有报道,但VDAC家族其他成员是否参与植物免疫反应还未见报道。本研究以拟南芥相关T-DNA插入突变体为材料,研究了VDAC3基因在植物抗病反应中的功能。病原相关分子模式flg22诱导的活性氧产生和胼胝质沉积在VDAC3突变体中都显著增强,表明VDAC3参与了病原相关分子模式触发的免疫反应。此外,效应因子激发的超敏性细胞死亡在vdac3突变体中也更明显,显示VDAC3也参与了效应子触发的免疫反应。以上结果说明,VDAC3在多个层面参与植物的抗病反应。
The mitochondrial outer membrane protein voltage-dependent anion channel (VDAC) is a key component of animal cell apoptosis regulatory system, but its function in plants is not yet clear. There are four members of the VDAC family of Arabidopsis thaliana, both of which can be induced by pathogens. The role of VDAC1 in hypersensitive cell death caused by pathogens has been reported. However, it has not been reported whether other VDAC family members participate in plant immune response . In this study, Arabidopsis T-DNA insertion mutants were used as materials to study the function of VDAC3 gene in plant disease resistance. Pathogen-associated molecular patterns flg22-induced ROS production and callose deposition were significantly increased in VDAC3 mutants, indicating that VDAC3 is involved in pathogen-associated molecular-triggered immune responses. In addition, effector-stimulated hypersensitivity cell death was also more evident in the vdac3 mutant, indicating that VDAC3 is also involved in effector-triggered immune responses. The above results indicate that VDAC3 is involved in plant disease resistance at multiple levels.