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目的:探讨补充肌酸对生长发育期雌、雄大鼠内源性肌酸合成的影响,为不同群体合理使用肌酸提供实验依据。方法:21天龄雌性和雄性SD大鼠随机分为5组,每组雌、雄大鼠各6或7只,分别连续补充0(对照液)、0.15、0.35、0.75或1.5g/kg/d肌酸液8周。观察内源性肌酸合成代谢途径中L-精氨酸-甘氨酸脒基转移酶(AGAT)活性和其它主要指标的变化。结果:与相同性别对照组相比,雌性或雄性大鼠补充肌酸8周后,肾脏AGAT活性和肝脏胍乙酸含量均降低,且补充肌酸剂量越大,降低越明显;腓肠肌、跖肌和比目鱼肌肌酸含量、血清肌酐含量升高;肾脏结构无明显差异。补充相同剂量肌酸的雄性大鼠肾脏AGAT活性和肝脏胍乙酸含量均高于雌性大鼠。结论:补充肌酸对生长发育期雌性或雄性大鼠的内源性肌酸合成均有抑制作用。雌性大鼠内源性肌酸合成能力弱于雄性。补充肌酸有助于提高骨骼肌肌酸含量。大剂量(1.5g/kg/d)补充肌酸不会对肾脏结构产生明显影响。
Objective: To investigate the effect of creatine supplementation on endogenous creatine synthesis in female and male rats during growth and development, and to provide experimental basis for different groups to use creatine properly. Methods: The 21-day-old female and male SD rats were randomly divided into 5 groups, each containing 6 or 7 male and female rats. The rats were continuously supplemented with 0 (control), 0.15, 0.35, 0.75 or 1.5 g / kg / d Creatine fluid for 8 weeks. To observe the changes of L-arginine-glycine amidotransferase (AGAT) activity and other major indexes in endogenous creatine metabolic pathway. Results: Compared with the same gender control group, the AGAT activity in kidney and the content of guanidinium acetate in liver of female or male rats decreased after 8 weeks of supplementation, and the greater the dose of creatine supplementation was, the more obvious the decrease was. The gastrocnemius and plantar muscle Creatine content of soleus, serum creatinine increased; no significant difference in the structure of the kidney. AGAT activity in kidney and guanidinium acetate in liver of male rats supplemented with the same dose of creatine were higher than those in female rats. CONCLUSION: Creatine supplementation inhibits endogenous creatine synthesis in both female and male rats during growth and development. The endogenous creatine synthesis capacity of female rats is weaker than that of males. Creatine supplementation helps to increase skeletal creatine levels. High doses (1.5g / kg / d) of creatine supplementation did not significantly affect the renal structure.