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目的了解分析牡丹江市2013-2014年流行性感冒(简称流感)病毒流行状况,为流感防控提供科学依据。方法通过全国流感监测网络系统收集2013-2014年牡丹江市流感病例资料。对4~9月各医院送检的流感咽试子样品采用Real Time RT-PCR方法进行检测;10月~次年3月咽试子样品采用MD-CK细胞进行病毒分离,血凝集抑制试验(HIA)进行病毒型别鉴定。结果牡丹江市2013-2014年流感样病例年龄构成以20~59岁组最高,占28.45%;其次为5~14岁组及15~24岁组,分别占25.63%和20.88%;60~岁组最低,仅占5.94%。共检测流感样病例咽拭子标本1 524份,其中新甲型H1N1型61份,占83.56%;甲型H3N2亚型3份,占4.11%;乙型9份,占12.33%。流行病学和病原学监测结果基本一致,均在1月达到顶峰。结论牡丹江市2013-2014年新甲型H1N1流感病毒占主要优势,未来防控重点为新甲型H1N1以外亚型,重点防控人群为5~24岁组的儿童和青少年。
Objective To understand and analyze the prevalence of influenza in Mudanjiang City from 2013 to 2014, and provide a scientific basis for prevention and control of influenza. Methods The data of influenza cases in Mudanjiang City from 2013 to 2014 were collected through the National Influenza Surveillance Network System. The real-time RT-PCR method was used to detect the samples of influenza pharynx in hospitals from April to September. The pharyngeal samples from March to March of the following year were collected for virus isolation and hemagglutination inhibition test (HIA) for virus type identification. Results The age of influenza-like illness cases in 2013-2014 in Mudanjiang City was the highest (28.45%) in the age group of 20 to 59 years, followed by the age group of 5 to 14 years and the age group of 15 to 24 years (25.63% and 20.88%, respectively) The lowest, only 5.94%. A total of 1 524 throat swab specimens were detected, of which 61 were new type A H1N1, accounting for 83.56%; 3 were type A H3N2, accounting for 4.11%; 9 were type B, accounting for 12.33%. Epidemiological and etiological monitoring results are basically the same, both peaked in January. Conclusion The new influenza A (H1N1) virus in Mudanjiang City from 2013 to 2014 accounted for the main advantage. In the future, the prevention and control focus should be on subtypes other than the new type A H1N1, especially in children and adolescents aged 5 to 24 years.