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神经生长因子(NGF)是由一连串氨基酸组成的蛋白质。Cohen 和Levi-Montalc-ini(1954)(1956)先后从鼠肉瘤180和蛇毒液中提取NGF,后来Levi-Montalcini(1958)又发现啮齿类动物的颌下腺中NGF活性最大,比从蛇毒液中提取的NGF 活性大10倍,比从鼠肉瘤180提取的NGF 活性大10,000倍,并证明NGF 有刺激和促进交感神经元的生长和生存作用。本实验目的是体外培养新生仔鼠的颈上神经节,在加入NGF 情况下,切断生长的神经纤维,观察NGF 对切断的神经纤维再生的影响。
Nerve growth factor (NGF) is a protein consisting of a series of amino acids. Cohen and Levi-Montalc-ini (1954) (1956) successively extracted NGF from murine sarcoma 180 and snake venom, and later Levi-Montalcini (1958) found that rodents had the largest NGF activity in the submandibular glands compared to snake venom 10-fold more NGF activity and 10,000-fold greater activity than NGF extracted from murine sarcoma 180, demonstrating that NGF stimulates and promotes the growth and survival of sympathetic neurons. The purpose of this experiment is to culture neonatal pups in the upper cervical ganglion, with the addition of NGF, cut off the growth of nerve fibers to observe the nerve fibers of NGF cut off the impact of regeneration.