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目的探讨肿瘤转移相关基因1(MTA1)和肿瘤转移抑制基因1(KAI1)在声门上型喉癌组织中的表达及其与临床病理特征的关系。方法选取60例声门上型喉癌患者手术切除的病理组织标本(实验组)及20例癌旁组织标本(对照组),应用免疫组织化学法检测MTA1和KAI1蛋白的表达。结果实验组MTA1蛋白表达阳性率高于对照组(P<0.05),KAI1蛋白表达阳性率低于对照组(P<0.05);实验组MTA1蛋白阳性表达率38.3%,明显高于于对照组的0%,且与喉癌组织分级、临床分期和淋巴结转移关系密切(P<0.05);实验组KAI1蛋白阳性表达率为40.0%,明显低于对照组的75.0%,且与喉癌的淋巴结转移密切相关(P<0.05);但在不同年龄、性别、临床分期及组织分级的患者之间,表达差异无统计学意义(P>0.05)。喉癌组织中MTAl蛋白与KAI1蛋白表达呈负相关(P<0.05)。结论 MTA1和KAI1的表达在声门上型喉癌的淋巴结转移过程中具有一定作用,可作为预测肿瘤转移潜能指标。
Objective To investigate the expression of metastasis-associated gene 1 (MTA1) and metastasis-suppressor gene 1 (KAI1) in supraglottic laryngeal carcinoma and its relationship with clinicopathological features. Methods Totally 60 pathological specimens of the supraglottic laryngeal carcinoma (experimental group) and 20 paracancerous tissues (control group) were collected. The expression of MTA1 and KAI1 protein were detected by immunohistochemistry. Results The positive rate of MTA1 protein in experimental group was higher than that in control group (P <0.05), and the positive rate of KAI1 protein expression in experimental group was lower than that in control group (P <0.05). The positive rate of MTA1 protein in experimental group was 38.3% (P <0.05). The positive expression rate of KAI1 protein in the experimental group was 40.0%, which was significantly lower than that in the control group (75.0%), and was significantly correlated with the lymph node metastasis of laryngeal carcinoma (P <0.05). However, there was no significant difference in the patients with different age, gender, clinical stage and histological grade (P> 0.05). There was a negative correlation between MTA1 protein and KAI1 protein expression in laryngeal carcinoma (P <0.05). Conclusion The expression of MTA1 and KAI1 plays a role in the process of lymph node metastasis of supraglottic laryngeal carcinoma and may be used as an index to predict tumor metastasis potential.