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目的探讨肿瘤坏死因子-α(TNF-α)对肺泡Ⅱ型上皮细胞株A549增殖及凋亡的影响。方法将2003年2月至2004年1月中国医科大学附属第一医院放射肿瘤科实验室的肺泡Ⅱ型上皮细胞株A549用TNF-α处理后计算存活细胞数,四甲基偶氮唑盐(MTT)法测定细胞增殖能力,流式细胞仪检测细胞凋亡。结果TNF-α浓度和时间依赖性地抑制肺泡上皮细胞的增殖;TNF-α培养肺泡Ⅱ型上皮细胞12~48h后,凋亡的细胞数(%)明显高于对照组。结论TNF-α通过抑制肺泡Ⅱ型上皮细胞增殖和促进其凋亡,损伤肺泡上皮细胞,导致肺损伤。
Objective To investigate the effects of tumor necrosis factor-α (TNF-α) on the proliferation and apoptosis of alveolar type Ⅱ epithelial cell line A549. Methods From February 2003 to January 2004, the alveolar type II epithelial cell line A549 in the Radiation Oncology Laboratory of the First Affiliated Hospital of China Medical University was treated with TNF-α to calculate the number of surviving cells, MTT assay was used to determine the cell proliferation and flow cytometry was used to detect apoptosis. Results The concentration of TNF-α inhibited the proliferation of alveolar epithelial cells in a time-dependent manner. The number of apoptotic alveolar type Ⅱ cells (%) after cultured with TNF-α for 12-48 hours was significantly higher than that of the control group. Conclusion TNF-α can inhibit the proliferation and promote the apoptosis of alveolar type Ⅱ epithelial cells, injure the alveolar epithelial cells and lead to lung injury.