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Based on single-factor experiment and L9( 34) orthogonal experiment,a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. was established and optimized. The results indicated that the optimal concentrations of various components in a 20 μl ISSR-PCR reaction system of S. przewalskii Maxim. were: 2μl of 10 × buffer( Mg2 +),0. 4 mmol/L d NTPs,1. 0 U of Taq DNA polymerase,0. 3 μmol/L ISSR primers and 30 ng of DNA template. This study laid the foundation for the utilization of S. przewalskii Maxim. germplasm resources.
Based on single-factor experiment and L9 (34) orthogonal experiment, a stable ISSR-PCR reaction system of Salvia przewalskii Maxim. Was established and optimized. The results indicated that the optimal concentrations of various components in a 20 μl ISSR-PCR reaction system of U. przewalskii Maxim. were: 2 μl of 10 × buffer (Mg 2+), 0.4 mmol / L d NTPs, 1.0 U of Taq DNA polymerase, 0.3 μmol / L ISSR primers and 30 ng of DNA template . This study laid the foundation for the utilization of S. przewalskii Maxim. Germplasm resources.