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目的在对4种卷柏类药材中2种特征性成分selaginellin和穗花杉双黄酮进行结构确证的基础上,首次建立同时测定此2种成分含量的HPLC方法。方法采用岛津VP-ODS色谱柱(4.6mm×250mm,5μm),流动相为乙腈-0.2%醋酸水溶液,梯度洗脱,流速为1.2mL·min-1,检测波长为432和338nm,柱温为30℃。结果在此色谱条件下,selaginellin和穗花杉双黄酮得到完全分离,分别在0.025～1.016和0.221～8.853μg内线性良好(r均为1.0000),平均回收率分别为98.64%和99.34%,RSD分别为0.86%和0.94%(n=6)。结论Selaginellin为首次从垫状卷柏中分离得到。含量分析方法操作简单、快速、准确,灵敏度高,重现性好,可为卷柏类药材质量评价和控制提供参考;对4种卷柏类药材进行了测定,所测样品含量差异较大。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of two components, selaginellin and biflavonoids from four kinds of Selapa drugs. Methods The mobile phase was acetonitrile-0.2% acetic acid aqueous solution with gradient elution at a flow rate of 1.2 mL · min-1 using the Shimadzu VP-ODS column (4.6 mm × 250 mm, 5 μm) with detection wavelength at 432 and 338 nm. Is 30 ° C. Results Under the chromatographic conditions, selaginellin and biflavum flavone were completely separated, with good linearity (r = 1.0000) in the range of 0.025-1.016 and 0.221-8.853μg, respectively. The average recoveries were 98.64% and 99.34% 0.86% and 0.94% (n = 6). Conclusions Selaginellin was isolated for the first time from the genus Selaginella. Content analysis method is simple, rapid, accurate, sensitive and reproducible. It can provide reference for the quality evaluation and control of Selaginella .And four kinds of Selapa drugs were determined. The content of the tested samples varied greatly.