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目的研究双歧杆菌表面分子细胞壁肽聚糖（ＷＰＧ）、脂磷壁酸（ＬＴＡ）对ＬＰＳ体内诱导小鼠胸腺细胞凋亡的调节。方法用ＤＮＡ凝胶电泳、ＴＵＮＥＬ法检测ＷＰＧ、ＬＴＡ对ＬＰＳ体内诱导小鼠胸腺细胞凋亡的影响，并分别用生物活性法和Ｇｒｉｅｓｓ反应测定ＷＰＧ、ＬＴＡ、ＬＰＳ体外诱生ＴＮＦ－α、ＮＯ２－的含量。结果ＷＰＧ、ＬＴＡ可显著抑制ＬＰＳ体内诱导的小鼠胸腺细胞的凋亡。ＷＰＧ、ＬＴＡ单独刺激巨噬细胞时所诱生的ＴＮＦ－α、ＮＯ的量显著低于ＬＰＳ刺激时所产生的这两种活性介质的量，而ＷＰＧ、ＬＴＡ与ＬＰＳ共同应用时可显著降低ＬＰＳ诱导巨噬细胞产生的ＴＮＦ－α、ＮＯ的量；诱生型一氧化氮合成酶抑制剂Ｓ－甲基异硫脲硫酸盐体外可抑制巨噬细胞产生的ＮＯ的量，在体内可部分抑制ＬＰＳ诱导的小鼠胸腺细胞的凋亡。结论ＷＰＧ、ＬＴＡ与ＬＰＳ共同应用时，可抑制ＬＰＳ诱导的巨噬细胞产生的ＴＮＦ－α、ＮＯ的量，从而下调ＬＰＳ体内诱导小鼠胸腺细胞的凋亡
Objective To study the regulation of apoptosis of thymocytes induced by lipopolysaccharide (LPS) induced by lipopolysaccharide (WPG) and lipoteichoic acid (LTA) on Bifidobacterium in vivo. Methods The effects of WPG and LTA on the apoptosis of thymocytes induced by LPS in mice were detected by DNA gel electrophoresis and TUNEL assay. The biological activity and Griess reaction were used to detect the expressions of TNF-α and NO2- Content. Results WPG and LTA significantly inhibited the apoptosis of mouse thymocytes induced by LPS in vivo. The amounts of TNF-α and NO induced by WPG and LTA alone stimulated macrophages were significantly lower than those produced by LPS stimulation, whereas WPG and LTA combined with LPS significantly decreased LPS Induced the amount of TNF-α and NO produced by macrophages; S-methyl isothiourea sulfate, an inducible inhibitor of nitric oxide synthase, inhibited the amount of NO produced by macrophages in vitro and was partially inhibited in vivo LPS-induced apoptosis in mouse thymocytes. Conclusion WPG, LTA combined with LPS can inhibit the LPS-induced macrophages produce TNF-α, NO, thereby reducing the LPS induced thymocyte apoptosis in mice