为了探讨人乳头瘤病毒感染与阴茎肿瘤的关系。用人乳头瘤病毒基因通用引物行聚合酶链反应，检测５２例阴茎鳞癌。 ＨＰＶ阳性率为６７％， ３１例为ＨＰＶ－１６， ３例为ＨＰＶ１６／１８， １例为ＨＰＶ１１／１８。 ４例阴茎癌淋巴结转移灶标本，３例检出与原发癌一致的ＨＰＶ．ＤＮＡ。 ６例阴茎乳头状瘤标本， ４例检出ＨＰＶ－６或１１。 ２０例正常阴茎包皮均阴性。提示ＨＰＶ在阴茎肿瘤的发生发展中有一定的作用，但与肿瘤的病理分级无关。 To investigate the relationship between human papillomavirus infection and penile tumor. Fifty-two penis squamous cell carcinomas were detected by polymerase chain reaction using universal primers for human papillomavirus gene. The HPV positive rate was 67%, 31 were HPV-16, 3 were HPV 16/18, and 1 was HPV 11/18. In 4 cases of lymph node metastasis of penis cancer, HPV was detected in 3 cases consistent with primary cancer. DNA. Six cases of penile papilloma tumors were detected in 4 cases and HPV-6 or 11 were detected in 4 cases. 20 cases of normal penis prepuce were negative. It suggests that HPV has a certain role in the development of penile tumors, but has nothing to do with the pathological grade of the tumor.