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目的探讨人类表皮生长因子受体2(HER2)和第10号染色体同源缺失性磷酸酶-张力蛋白基因(PTEN)在胃癌组织中的表达及其与临床病理特征的关系。方法收集有效胃癌组织蜡块407例,在胃癌组织芯片上,应用免疫组化EnVision法检测HER2和PTEN蛋白表达水平,其结果与30例正常胃黏膜组织比较。分析HER2和PTEN在胃癌组织的表达与患者临床病理特征的关系。结果 HER2在胃癌组织中的阳性表达率为9.1%;PTEN在胃癌中的阳性表达率为20.1%,显著低于正常胃黏膜组织的100%(P<0.01)。HER2的表达与肿瘤分化程度、组织学Lauren′s分型相关(P<0.05);PTEN的表达与组织分化程度、组织学分型、淋巴结转移相关(P<0.05);HER2与PTEN的表达呈负相关(r=-0.344,P<0.01)。结论 PTEN蛋白的表达与胃癌的发生、发展密切相关;同时检测胃癌组织中的HER2和PTEN蛋白的表达对判断肿瘤的恶性程度和预后可能有一定意义。
Objective To investigate the expression of human epidermal growth factor receptor 2 (HER2) and chromosome 10 homologous deletion phosphatase-tensin gene (PTEN) in gastric carcinoma and its relationship with clinicopathological features. Methods 407 cases of effective gastric cancer tissue paraffin blocks were collected. The expression of HER2 and PTEN protein was detected by EnVision immunohistochemistry in gastric cancer tissue microarray. The results were compared with those of 30 normal gastric mucosa. To analyze the relationship between the expression of HER2 and PTEN in gastric cancer and clinicopathological features. Results The positive rate of HER2 in gastric cancer was 9.1%. The positive rate of PTEN in gastric cancer was 20.1%, which was significantly lower than that in normal gastric mucosa (P <0.01). The expression of HER2 was correlated with the degree of tumor differentiation and Lauren’s histological classification (P <0.05). The expression of PTEN was correlated with the degree of histological differentiation, histological type and lymph node metastasis (P <0.05). The expression of HER2 and PTEN was negative (R = -0.344, P <0.01). Conclusions The expression of PTEN protein is closely related to the occurrence and development of gastric cancer. Simultaneously detecting the expression of HER2 and PTEN in gastric cancer may be of significance in judging the malignant degree and prognosis of gastric cancer.