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该文建立了HPLC同时测定狭基线纹香茶菜水溶性总黄酮中6种碳苷黄酮含量的方法,并对狭基线纹香茶菜水溶性总黄酮进行抗肿瘤活性评价。采用Kromasil 100-5 C_(18)(4.6 mm×250 mm,5μm)色谱柱;流动相为甲醇-乙腈-0.5%甲酸梯度洗脱;体积流量0.8 mL·min~(-1),检测波长334 nm;柱温为室温(25℃)。以HepG2细胞为供试细胞株,采用MTT法评价水溶性总黄酮的抗肿瘤活性。新西兰牡荆苷2、新西兰牡荆苷3、异夏佛塔苷、夏佛塔苷、牡荆苷和芹菜素-6,8-二-C-α-L-吡喃阿拉伯糖苷线性范围分别为0.25~2.53,0.12~1.20,0.37~3.69,0.16~1.63,0.19~1.92,0.14~1.42μg;平均回收率(n=6)分别为99.6%,100.2%,99.6%,97.9%,98.8%,98.6%,RSD分别为0.87%,2.0%,1.8%,1.5%,1.2%,1.2%。以IC_(50)值作为评价指标,测得狭基线纹香共菜水溶性总黄酮在给药24,48,72 h后抑制HepG2细胞增殖的IC_(50)值分别为1.89,1.71,1.51g·L~(-1)。本法快速、简便、准确,重复性好,可用于狭基线纹香共菜水溶性总黄酮的质量控制。狭基线纹香茶菜水溶性总黄酮对HepG2细胞增殖有一定的抑制作用。
A method for the simultaneous determination of six kinds of carbohydrate flavonoids in water-soluble flavonoids from Syringa obliquus L. was established by HPLC. The antitumor activity of water-soluble flavonoids from Syringa obliquus was evaluated. The mobile phase consisted of gradient elution with methanol-acetonitrile-0.5% formic acid. The volume flow rate was 0.8 mL · min -1 and the detection wavelength was 334 nm; column temperature is room temperature (25 ℃). HepG2 cells as test cell lines, the use of MTT evaluation of water-soluble flavonoids antitumor activity. The linear ranges of vitexin 2, vitexin 3, isatin, taxifolin, vitexin and apigenin-6,8-di-C-α-L-arabinopyranoside were 0.25 ~ 2.53,0.12 ~ 1.20,0.37 ~ 3.69,0.16 ~ 1.63,0.19 ~ 1.92,0.14 ~ 1.42μg, the average recoveries were 99.6%, 100.2%, 99.6%, 97.9% and 98.8% 98.6% with RSD of 0.87%, 2.0%, 1.8%, 1.5%, 1.2% and 1.2% respectively. The value of IC 50 was used as the evaluation index to determine the IC 50 of HepG2 cells inhibited by 24, 48 and 72 h after the administration of water-soluble flavonoids from R. solanacearum were 1.89, 1.71 and 1.51 g · L ~ (-1). This method is rapid, simple, accurate, reproducible and can be used for the quality control of water-soluble flavonoids in the common root-wrinkled Rhizome. The water-soluble flavonoids of the basilar line Syringa sativus L. had a certain inhibitory effect on the proliferation of HepG2 cells.