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目的:考察并比较厚朴酚与和厚朴酚对小鼠腹泻及胃肠排空抑制的影响。方法:将小鼠分为17组,每组8只,即正常对照组、模型组、盐酸洛哌丁胺(2 mg/kg)组、厚朴酚(5、10、15、20、25、30、40 mg/kg)组及和厚朴酚(5、10、15、20、25、30、40 mg/kg)组。分别比较厚朴酚与和厚朴酚对大黄致腹泻小鼠小肠炭末推进、番泻叶致小鼠腹泻、阿托品致小鼠胃排空抑制及肾上腺素负荷小鼠小肠推进抑制的影响。结果:对大黄致腹泻小鼠小肠炭末推进的影响:与正常对照组比较,模型组的炭末推进率显著升高(P<0.05),与模型组比较,厚朴酚5~40 mg/kg组及和厚朴酚15~40 mg/kg组炭末推进率显著降低(P<0.05);对番泻叶致小鼠腹泻的影响:与正常对照组比较,模型组的湿粪粒数显著增多(P<0.05),与模型组比较,厚朴酚10~40 mg/kg组及和厚朴酚15~40 mg/kg组湿粪粒数显著减少(P<0.05);对阿托品致小鼠胃排空抑制的影响:与正常对照组比较,模型组胃排空率显著降低(P<0.05),与模型组比较,厚朴酚15~40 mg/kg组及和厚朴酚20~40 mg/kg组胃排空率显著升高(P<0.05);厚朴酚与和厚朴酚对肾上腺素负荷小鼠小肠推进无明显影响作用。与和厚朴酚比较,厚朴酚对小肠炭末推进、对番泻叶致小鼠腹泻和对阿托品致小鼠胃排空抑制影响的EC50均较低。结论:厚朴酚与和厚朴酚均有抗腹泻作用,对大黄致腹泻小鼠小肠炭末推进、番泻叶致小鼠腹泻和阿托品致小鼠胃排空抑制均有明显的抑制效应,但对肾上腺素负荷小鼠小肠推进无明显影响,二者量效曲线相似,但厚朴酚的效能较高。
Objective: To investigate and compare the effects of honokiol and honokiol on the inhibition of diarrhea and gastrointestinal emptying in mice. Methods: The mice were divided into 17 groups with 8 rats in each group, namely normal control group, model group, loperamide hydrochloride (2 mg / kg), honokiol (5,10,15,20,25, 30, 40 mg / kg) and honokiol (5, 10, 15, 20, 25, 30, 40 mg / kg) The effects of honokiol and honokiol on intestinal charcoal propulsion in mice with diarrhea induced by rhubarb, diarrhea in mice induced by senna, inhibition of gastric emptying induced by atropine and inhibition of small intestine in mice treated with epinephrine were compared respectively. Results: The effect of small intestine charcoal propulsion on rhubarb-induced diarrhea mice: compared with the normal control group, the carbon propulsion rate in the model group was significantly increased (P <0.05). Compared with the model group, honokiol 5-40 mg / kg group and honokiol 15 ~ 40 mg / kg group was significantly reduced (P <0.05); the diarrhea of mice induced by senna: Compared with the normal control group, the model group of wet dung particles (P <0.05). Compared with the model group, the numbers of wet pellets in magnolol 10 ~ 40 mg / kg group and honokiol 15 ~ 40 mg / kg group decreased significantly (P <0.05) The gastric emptying inhibition in mice: Compared with the normal control group, the gastric emptying rate of the model group was significantly lower (P <0.05), compared with the model group, honokiol 15 ~ 40 mg / kg group and honokiol 20 The gastric emptying rate of ~ 40 mg / kg group was significantly increased (P <0.05); honokiol and honokiol had no significant effect on the small intestine propulsion of adrenaline-loaded mice. Compared with honokiol, honokiol on the small intestine charcoal propulsion, diarrhea caused by senna and mouse atropine-induced gastric emptying inhibition EC50 lower. CONCLUSION: Both honokiol and honokiol have anti-diarrhea effects, which have significant inhibitory effects on the progression of small intestine charcoal-induced diarrhea in mice caused by rhubarb, diarrhea induced by senna and atropine-induced gastric emptying in mice. However, there was no significant effect on the small intestine propulsion in adrenergic-loaded mice. The two dose-response curves were similar, but the efficacy of magnolol was higher.