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目的 :探讨唾液蔗糖酶活性与口腔肿瘤恶变之间的相互关系。方法 :采用邻甲苯胺测糖法 ,测定单位时间内唾液蔗糖酶水解蔗糖生成的葡萄糖量 ;在PH6 ,37℃条件下进行酶反应用双倒数作图法测出表观Km和表观Vm值 ;检测蔗糖酶活性。结果 :正常人唾液蔗糖酶活性为 1 32 2± 0 434mU/mgpro ,口腔鳞癌患者唾液蔗糖酶活性为 1 5 48± 0 6 5 7mU/mgpro ,二者之间比较无显著差异 (P >0 0 5 ) ;正常人唾液蔗糖酶的表观Km =(1 1 2± 2 4)× 1 0 -2 mol/L ,表观Vm =1 1 38± 0 35 2 (每分钟光密度值 ) ,口腔鳞癌患者唾液蔗糖酶的表观Km =(9 5±2 2 )× 1 0 -2 mol/L ,表观Vm =(1 0 89± 0 6 32 ) (每分钟光密度值 )。结论 :口腔良性肿瘤、涎腺腺瘤、口腔鳞癌患者与正常人比 ,唾液蔗糖酶活性无显著差异。
Objective: To investigate the relationship between salivary sucrase activity and oral tumor malignancy. Methods: O-toluidine was used to measure the amount of glucose produced by sucrose hydrolysis of sucrose in unit time. The enzymatic reaction was carried out at pH6 and 37 ℃, and the apparent Km and apparent Vm were measured by double reciprocal mapping The activity of sucrase was tested. Results: The saliva sucrase activity of normal persons was 1 32 2 ± 0 434 mU / mgpro, and the salivary sucrase activity of oral squamous cell carcinoma patients was 15 48 ± 0 657 mU / mgpro. There was no significant difference between the two groups (P> 0 The apparent Km of normal human salivary sucrase was (112 ± 2 4) × 10 2 mol / L and the apparent Vm was 1138 ± 0 35 2 (optical density per minute) The apparent Km of salivary sucrase in patients with oral squamous cell carcinoma was (95 ± 2 2) × 10 -2 mol / L and the apparent Vm was (10 89 ± 0 6 32) (optical density per minute). Conclusion: There is no significant difference in salivary sucrase activity between oral benign tumor, salivary gland adenoma and oral squamous cell carcinoma compared with normal people.