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目的 观察电化学治疗对人肝癌细胞SMMC772 1化疗耐药性的影响 ,为其临床应用提供实验依据。方法 细胞计数法观察ECT、化疗药阿霉素 (ADR )结合的抑癌效应 ,应用“多药效应分析软件”分析两者之间的相互作用。流式细胞仪 (FCM )分析ECT对细胞内ADR累积的影响 ,免疫组化法检测ECT对SMMC772 1细胞P gp的影响 ,RT PCR法观察ECT对细胞MDR1mRNA水平的影响。 结果 ECT与ADR结合治疗的合并指数 (CI) >1,提示ECT、ADR呈拮抗效应 ,ECT使化疗耐药性增加 ,敏感性下降。ECT 5V1C处理后 2hSMMC772 1细胞内ADR的积累减少 46 .5 % ,ECT 5V2C处理后 2d细胞的MDR1mRNA及P gp表达水平升高。 结论 ECT上调SMMC772 1细胞MDR1基因的表达 ,增加化疗耐药性。
Objective To observe the effect of electrochemical treatment on chemoresistance of human hepatocellular carcinoma cell line SMMC772 1 and to provide experimental basis for its clinical application. Methods The cell counting method was used to observe the tumor suppressor effect of ECT and chemotherapeutic drug adriamycin (ADR). The interaction between ECT and chemotherapeutic drug adriamycin (ADR) was analyzed by using the multi-drug analysis software. The effect of ECT on the accumulation of intracellular ADR was analyzed by flow cytometry (FCM). The effect of ECT on P gp expression in SMMC772 1 cells was detected by immunohistochemistry. The effect of ECT on MDR1 mRNA expression was observed by RT PCR. Results The combination index (CI)> 1 of ECT combined with ADR suggested that ECT and ADR were antagonistic. ECT increased chemoresistance and decreased sensitivity. The accumulation of ADR in SMMC772 1 cells decreased by 46.5% at 2h after ECT treatment with 5V1C, and the expression of MDR1 mRNA and P gp increased 2 days after ECT treatment with 5V2C. Conclusion ECT up-regulates the expression of MDR1 gene in SMMC772 1 cells and increases chemoresistance.