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目的:研究地卓西平对吗啡依赖大鼠部分脑区突触素ⅠmRNA表达的影响。方法:雄性SD大鼠24只,随机等分为2组。吗啡组大鼠腹腔注射盐酸吗啡,2次/d,起始剂量5mg/kg,逐d递增5mg/kg,至第10d达50mg/kg;干预组大鼠在每次注射吗啡(同吗啡组)前30min腹腔注射地卓西平0.075mg/kg。末次注射后3h及72h,2组各取6只大鼠取脑并冰冻切片,留取中脑腹侧被盖区(VTA)、伏隔核(NAc)、中脑导水管灰质(PAG)、杏仁核(AMG)、海马CA1区(HIPCA1)的脑片,利用原位杂交技术检测突触素ⅠmRNA的表达。结果:末次注射后3h,干预组大鼠AMG、HIPCA1突触素ⅠmRNA的表达高于吗啡组;72h时,干预组大鼠NAc、HIPCA1突触素ⅠmRNA的表达高于吗啡组(P均<0.05)。其他脑区2组间差异无统计学意义(P>0.05)。结论:地卓西平可促进吗啡依赖大鼠部分脑区突触素Ⅰ基因的表达,这可能是其抑制吗啡依赖效应的机制之一。
Objective: To study the effect of Decoxib on the synaptophysin Ⅰ mRNA expression in some brain regions of morphine dependent rats. Methods: Twenty-four male SD rats were randomly divided into two groups. Morphine group rats were intraperitoneally injected with morphine hydrochloride twice a day for 5 mg / kg starting dose of 5 mg / kg, up to 50 mg / kg on the 10th day. Rats in the intervention group were injected morphine (morphine group) 30 minutes before the injection of diltiazem 0.075mg / kg. At 3 h and 72 h after the last injection, 6 rats in each group were taken from the brain and frozen sections. VTA, NAc, PAG, The amygdala (AMG) and hippocampal CA1 (HIPCA1) brain slices were used to detect the expression of synaptophysin Ⅰ mRNA by in situ hybridization. Results: At 3h after the last injection, the expression of synaptophysin Ⅰ mRNA of AMG and HIPCA1 in the intervention group was higher than that of the morphine group. At 72h, the expression of synaptophysin Ⅰ mRNA of NAc and HIPCA1 in the intervention group was higher than that in the morphine group ). There was no significant difference in other brain regions between the two groups (P> 0.05). Conclusion: Dezocipine can promote synaptophysin Ⅰ gene expression in some brain regions of morphine dependent rats, which may be one of the mechanisms of its inhibition of morphine dependence.