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目的:研究大黄牡丹汤对葡聚糖硫酸钠诱导的溃疡性结肠炎(ulcerative colitis,UC)小鼠的治疗作用及其相关的贫血症的改善作用。方法:设正常组、模型组、美沙拉嗪组、大黄牡丹汤低剂量组、大黄牡丹汤中剂量组和大黄牡丹汤高剂量组,通过疾病活动指数(DAI)、结肠病理损伤评分、结肠髓过氧化物酶(myeloperoxidase,MPO)活性、外周血中性粒细胞数量、红细胞总数及血红蛋白含量观察大黄牡丹汤对UC小鼠的治疗效果及对炎症性肠病相关的贫血症的影响。结果:模型组小鼠DAI评分显著升高,结肠横切面显著增厚,大量炎症性细胞浸润,溃疡部位明显,隐窝和肠腺减少,病理组织学评分显著升高,外周血中性粒细胞显著增多,红细胞数减少,血红蛋白含量显著减少。美沙拉秦可降低UC小鼠DAI评分、组织病理学评分,但对UC小鼠结肠增厚、中性粒细胞增多及红细胞及血红蛋白减少作用不明显。大黄牡丹汤个剂量组均能降低DAI评分、组织病理学评分、结肠MPO活性。大黄牡丹汤中剂量组和高剂量组还可显著防止结肠组织增厚,维持其结构的完整,显著抑制外周血中性粒细胞的增多和提高红细胞数及血红蛋白含量。结论:大黄牡丹汤可以改善葡聚糖硫酸钠诱导的溃疡性结肠炎小鼠炎症状态及其贫血症。
Objective: To study the therapeutic effect of Rhubarb and Peony Decoction on dextran sodium sulfate-induced ulcerative colitis (UC) mice and the related improvement of anemia. Methods: The normal control group, model group, mesalazine group, rhubarb and peony soup low-dose group, rhubarb and peony soup middle-dose group and rhubarb and peony soup high-dose group were divided into three groups according to disease activity index (DAI) The effect of rhubarb peony decoction on UC mice and its effect on inflammatory bowel disease-related anemia was observed by the activity of myeloperoxidase (MPO), the number of peripheral blood neutrophil, the total number of red blood cells and hemoglobin. Results: The DAI score of the model group was significantly increased, the cross-section of the colon was significantly thicker, a large number of inflammatory cells infiltrated, the site of the ulcer was obvious, the number of crypts and gut glands was decreased, the histopathological score was significantly increased, the level of peripheral blood neutrophil Significantly increased, reduced red blood cell count, hemoglobin content decreased significantly. Mesalazine can reduce the DAI score and histopathological score of UC mice, but have no obvious effect on the colon thickening, neutrophilic granulocyte and the decrease of red blood cells and hemoglobin in UC mice. Dahuang Peony Decoction dose group can reduce the DAI score, histopathological score, colon MPO activity. Rhubarb Mudan middle dose group and high dose group also significantly prevent the thickening of colon tissue to maintain its structural integrity, significantly inhibited the increase in peripheral blood neutrophils and increase the number of red blood cells and hemoglobin content. Conclusion: Rhubarb Peony Decoction can improve the dextran sodium sulfate-induced inflammatory state in mice with ulcerative colitis and its anemia.