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本研究旨在探讨外源性给予杏仁核中央核区不同浓度的降钙素基因相关肽(calcitonin gene-related peptide,CGRP)对小鼠认知和学习记忆的影响。将30日龄C57BL/6J小鼠随机分为空白对照组、手术对照组、200 ng CGRP组、400 ng CGRP组和800 ng CGRP组,每组10只。外源性给予双侧杏仁核中央核区不同浓度的CGRP后,用旷场实验检测各组小鼠认知功能,用新物体认知实验和Morris水迷宫实验检测各组小鼠学习记忆能力。旷场实验结果显示,与空白对照组相比,800 ng CGRP组小鼠的水平运动得分显著增加。新物体认知实验结果显示,与空白对照组相比,400 ng CGRP组的认知指数显著提高。Morris水迷宫实验结果显示,与空白对照组比较,400和800 ng CGRP组小鼠的潜伏期均显著缩短,穿越平台的次数显著增加;与200 ng CGRP组比较,400和800 ng CGRP组小鼠寻找平台的潜伏期均明显缩短。以上结果提示,双侧杏仁核注射CGRP可剂量依赖性促进小鼠非空间和空间学习记忆功能。
This study aimed to investigate the effects of exogenous administration of calcitonin gene-related peptide (CGRP) at different concentrations on the cognition and learning and memory of mice. The 30-day-old C57BL / 6J mice were randomly divided into blank control group, operation control group, 200 ng CGRP group, 400 ng CGRP group and 800 ng CGRP group, with 10 rats in each group. After exogenous administration of different concentrations of CGRP in the central nucleus of the bilateral amygdala, cognitive function of the mice in each group was tested by open-field test. The learning and memory abilities of mice in each group were detected by new object cognition test and Morris water maze test. Open-field test results showed that the level of exercise scores of 800 ng CGRP mice increased significantly compared with the blank control group. The new object cognitive experiment results showed that compared with the blank control group, the cognitive index of 400 ng CGRP group increased significantly. The results of Morris water maze test showed that compared with the blank control group, the latency of mice in 400 and 800 ng CGRP groups was significantly shortened and the number of crossing the platform significantly increased. Compared with 200 ng CGRP group, the mice in 400 and 800 ng CGRP groups looked for Platform latency were significantly shorter. These results suggest that BGR injection of CGRP can promote mice’s non-spatial and spatial learning and memory function in a dose-dependent manner.