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目的：观察急性肺损伤（ＡＬＩ）大鼠肺组织β、β２受体和β２受体ｍＲＮＡ含量的变化，探讨上述变化与ＡＬＩ的关系及β２受体变化可能的分子机制。方法：静注内毒素复制大鼠ＡＬＩ模型，肺组织β、β２受体及β２受体ｍＲＮＡ含量，分别用放射性配基结合分析法和斑点杂交技术测定。结果：静注内毒素后１、４、６ｈ，大鼠肺组织β和β２受体的最大结合容量（Ｂｍａｘ）均明显低于正常对照组（Ｐ＜０．０１），尤以β２受体为甚；静注内毒素后４及６小时，大鼠肺组织β２受体ｍＲＮＡ含量分别下降至正常对照组的７６４％±１９６％（Ｐ＜０．０１）和５２８％±２０４％（Ｐ＜０．０１）。结论：肺组织β受体数目的减少在ＡＬＩ发生发展中可能起一定的作用，β２受体数目的减少可能与ＡＬＩ关系更密切；β２受体ｍＲＮＡ含量的减少可能是ＡＬＩ后一定阶段肺组织β２受体数目减少的原因之一。
OBJECTIVE: To observe the changes of mRNA expression of β, β2 and β2 receptors in the lungs of rats with acute lung injury (ALI) and to explore the relationship between these changes and ALI and the possible molecular mechanism of β2 receptors. Methods: The ALI model of endotoxin-induced ALI rat model was established. The contents of β, β2 and β2 receptor mRNA in lung tissue were determined by radioligand binding assay and dot blot hybridization. Results: The maximal binding capacity of β and β2 receptors in lung tissue of rats at 1, 4 and 6 h after endotoxin was significantly lower than that of normal control group (P <0.01), especially β2 receptor was (P <0.01), and the level of β2 receptor mRNA in lung tissue decreased to 764% ± 196% (P <0.01) and 528% ± in normal control group at 4 and 6 hours after intravenous injection of endotoxin 20.4% (P <0.01). CONCLUSIONS: The decreased number of β receptors in lung tissue may play a role in the development of ALI. The decrease of β2 receptors may be more closely related to ALI. The reduction of β2 receptor mRNA may be the result of the change of β2 One of the reasons for the decrease in the number of receptors.