论文部分内容阅读
目的研究自发性遗传性癫痫大鼠(Tremor,TRM)小脑组织中CaV1.2与钙调蛋白(Calmodulin,CaM)蛋白表达与共定位情况。方法应用Westernblot技术检测CaV1.2与CaM蛋白表达,应用免疫荧光技术定位CaV1.2与CaM蛋白的分布,应用激光共聚焦显微镜检测[Ca2+]。结果 TRM大鼠小脑内CaV1.2的蛋白表达低于正常iWistar大鼠(P<0.05),而CaM的蛋白表达高于正常Wistar大鼠(P<0.05);荧光定位显示CaV1.2与CaM共定位的阳性细胞比率较之正常组表达降低(P<0.01)。与正常的Wistar大鼠相比,急性分离的TRM大鼠小脑神经元中[Ca2+]明显增强。结论 TRM大鼠小脑内CaV1.2和CaM蛋白表达异常可能是癫痫的发病机制之一。
Objective To study the expression and co-localization of CaV1.2 and Calmodulin (CaM) in cerebellar tissues of spontaneous genetic epilepsy rats (Tremor, TRM). Methods Western blotting was used to detect the expression of CaV1.2 and CaM protein. The distribution of CaV1.2 and CaM protein was detected by immunofluorescence technique. [Ca2 +] was detected by laser confocal microscopy. Results The protein expression of CaV1.2 in cerebellum of TRM rats was lower than that of normal Wistar rats (P <0.05), while the protein expression of CaM was higher than that of normal Wistar rats (P <0.05). The fluorescence localization of CaV1.2 The positive rate of localization was lower than that of the normal group (P <0.01). Compared with normal Wistar rats, [Ca2 +] in cerebellar neurons of acutely isolated TRM rats was significantly increased. Conclusion The abnormal expression of CaV1.2 and CaM in the cerebellum of TRM rats may be one of the pathogenesis of epilepsy.