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因发现儿童大脑皮层内的微血管内皮是粗糙的 ,象树皮样 ,故提出血液缘流保护性屏障假说。为验证这一假说 ,在 18例经 1∶6福尔马林与碳素墨水混合液灌注的儿童尸头的大脑半球光学切片上观察了大脑皮层微血管的形态特征 ,发现大脑皮层内存在着 4种微血管 :大脑皮层浅动脉、中动脉、深动脉和皮质髓质动脉。在扫描电镜下观察了 10例经甲基丙烯酸甲酯灌注的儿童尸头的大脑皮层微血管构筑腐蚀筑型 ,发现儿童大脑皮层内的微血管内皮是粗糙的 ,形态就象树皮。激光扫描共聚焦显微镜下观察家兔大脑皮层内微循环 ,血浆被用荧光素标记后 ,镜下可无损伤动态观察大脑皮层下 30 0 μm的大脑皮层内微循环。在给予川芎嗪和去甲基肾上腺素引起大脑皮层内血管口径变化的血管内的血液轴流和缘流的荧光灰度被图象分析系统采取 ,并绘出荧光灰度曲线。在正常状态下 ,显示口径为 12 1.5 6 μm大脑皮层内血管在舒缩运动时血液缘流的厚度没有减少 (P >0 .0 5 ) ;口径为 6 0 .0 0 μm大脑皮层内血管在舒缩运动时血液缘流的厚度没有减少 (P >0 .0 5 ) ;在休克状态下口径为 6 0 .15 μm大脑皮层内血管在舒缩运动时血液缘流的厚度没有减少 (P >0 .0 5 ) ;口径为 4 8.84 μm大脑皮层内血管在舒缩运动时血液缘流的厚度没有减少 (P >0 .0
Because of the discovery that the microvascular endothelium in children’s cerebral cortex is rough, like bark-like, a hypothesis of protective blood-flow barrier was proposed. To verify this hypothesis, morphological features of cerebral cortex microvessels were observed on optical sections of the cerebral hemispheres of 18 cadaveric heads of children injected with 1: 6 formalin and carbon ink mixture and found that there were 4 Microvessels: Cerebral cortex superficial, middle, deep and cortical medulla. Scanning electron microscopy of 10 cases of methyl methacrylate perfused cadaveric cortex capillaries build erosive build, found that the microvascular endothelium in children’s cerebral cortex is rough, the shape of the bark. The microcirculation in the cerebral cortex of rabbits was observed by laser scanning confocal microscopy. The plasma was labeled with fluorescein, and the microcirculation in the cerebral cortex under 30 μm in the cerebral cortex was observed dynamically under the microscope. Fluorescent grayscales in the blood vessels within the blood vessels that give rise to ligustrazine and norepinephrine changes in blood vessels within the cerebral cortex are taken by the image analysis system and the fluorescence grayscale curve is plotted. Under normal conditions, blood flow in the cerebral cortex with a caliber of 12 1.5 6 μm showed no decrease in the thickness of blood flow during exercise (P> 0.05); in the caliber of 60.0 μm, There was no decrease in the thickness of blood flow during systolic and diastolic movement (P> 0.05). The thickness of blood flow in the cerebral cortex with diameter of 60.15 μm during shock and contraction was not decreased in shock state (P> 0 .0 5). The thickness of blood flow in the cortex with caliber 4 8.84 μm did not decrease in the systolic and diastolic movement (P> 0.05)