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目的探讨丙泊酚后处理对大鼠肝缺血再灌注损伤的影响。方法成年健康Wistar大鼠60只,随机分为5组(n=12):假手术组(S组)、缺血再灌注组(IR组)、丙泊酚1、2、4mg/kg后处理组(P1、P2、P4组)。用逆转录-聚合酶链反应(RT-PCR)法测定肝组织中TLR4 mRNA、NF-κB mRNA的表达,放射免疫法测定血清TNF-α的浓度,光镜观察肝组织形态学变化。结果与S组比较,各组TLR4 mRNA、NF-κB mRNA表达上调,TNF-α升高,差别均有统计学意义(P<0.01);与IR组比较,P1、P2、P4组TLR4 mRNA、NF-κB mRNA表达下调,TNF-α降低(P<0.01);与P4组比较,P1、P2组TLR4 mRNA、NF-κB mRNA表达下调,TNF-α降低(P<0.01);与P1组比较,P2组TLR4 mRNA、NF-κB mRNA表达下调,TNF-α降低(P<0.01)。病理检查显示P1、P2、P4组肝组织结构损伤明显小于IR组。结论丙泊酚后处理减轻大鼠肝缺血再灌注损伤的机制可能与下调TLR4、NF-κB的表达,降低肝组织炎症反应有关。
Objective To investigate the effect of propofol postconditioning on hepatic ischemia-reperfusion injury in rats. Methods Sixty adult healthy Wistar rats were randomly divided into 5 groups (n = 12): sham operation group (S group), ischemia reperfusion group (IR group), propofol 1,2,4 mg / kg postconditioning Group (P1, P2, P4 group). The expression of TLR4 mRNA and NF-κB mRNA in liver tissue were detected by reverse transcription-polymerase chain reaction (RT-PCR). The concentration of serum TNF-α was measured by radioimmunoassay. The morphological changes of liver tissue were observed by light microscope. Results Compared with S group, the expressions of TLR4 mRNA and NF-κB mRNA were up-regulated and TNF-α was increased in all groups (P <0.01). Compared with IR group, the expressions of TLR4 mRNA, (P <0.01). Compared with P4 group, the expressions of TLR4 mRNA and NF-κB mRNA were down-regulated and the level of TNF-α was down-regulated in group P1 and P2 (P <0.01) , While the expressions of TLR4 mRNA and NF-κB mRNA in P2 group were decreased and TNF-α was decreased (P <0.01). Pathological examination showed that the damage of liver tissue in P1, P2 and P4 groups was significantly less than that in IR group. Conclusion The mechanism of propofol postconditioning alleviating hepatic ischemia-reperfusion injury in rats may be related to down-regulating the expression of TLR4 and NF-κB and decreasing the inflammatory reaction in liver tissue.