论文部分内容阅读
目的研究细胞有丝分裂前期检查点(checkpoint with forkhead associated and ring finger,CHFR)在人肝癌细胞株HepG2中的表达,以及CHFR基因甲基化对HepG2细胞凋亡作用的影响。方法采用去甲基化剂5-氮-2-脱氧胞苷(5-Aza-2-deoxycytidine,Aza)处理人肝癌细胞株HepG2,半定量RT-PCR和Western-Blot检测CHFR在HepG2细胞中的表达水平,FCM分析HepG2细胞的凋亡情况。结果 CHFR在HepG2细胞中表达缺失;经Aza去甲基化处理后,CHFR在HepG2细胞中逐渐恢复表达,肿瘤细胞凋亡率逐渐增高,存在剂量-反应关系;且CHFR表达水平与HepG2细胞凋亡率呈正相关。结论在本试验条件下,CHFR在HepG2细胞中表达缺失,去甲基化后表达恢复;CHFR的高表达可以促进肝癌细胞凋亡。
Objective To investigate the expression of checkpoint with forkhead associated and ring finger (CHFR) in human hepatocellular carcinoma cell line HepG2 and the effect of CHFR gene methylation on the apoptosis of HepG2 cells. Methods HepG2 cells were treated with demethylation agent 5-Aza-2-deoxycytidine (Aza) and the expression of CHFR in HepG2 cells was detected by semi-quantitative RT-PCR and Western-Blot Expression level, FCM analysis of HepG2 cell apoptosis. Results The expression of CHFR was absent in HepG2 cells. After demethylation, the expression of CHFR gradually recovered in HepG2 cells, and the apoptosis rate of tumor cells was gradually increased with dose-response relationship. The expression of CHFR correlated with the apoptosis of HepG2 cells The rate was positively correlated. Conclusions The expression of CHFR in HepG2 cells is lost after demethylation and the expression of CHFR is restored under the experimental conditions. The high expression of CHFR can promote the apoptosis of hepatocellular carcinoma cells.