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目的探讨吞噬细胞运动蛋白1(engulfment and cell motility 1,ELMO1)在IL-8诱导的乳腺癌细胞侵袭和转移过程中的作用。方法采用趋化运动实验检测不同浓度IL-8刺激下乳腺癌细胞的趋化运动能力;采用Western blot检测乳腺癌细胞中ELMO1的表达情况;利用小RNA干扰技术,瞬时转染乳腺癌细胞MDA-MB-231,用过表达质粒上调乳腺癌细胞MCF-7中ELMO1的表达;应用趋化运动实验和Transwell侵袭实验检测各组转染细胞的趋化和侵袭能力。结果趋化运动实验结果显示,在IL-8刺激下,乳腺癌细胞MDA-MB-231和MCF-7的运动能力明显增强,具有剂量依赖性;Western blot结果显示ELMO1在si ELMO1/MDA-MB-231细胞中的表达明显降低,而在MCF-7/ELMO1细胞中的表达明显增高;趋化运动实验结果显示在IL-8刺激下,Si ELMO1/MDA-MB-231细胞组的趋化运动能力明显降低,MCF-7/ELMO1细胞组的趋化运动能力明显增强;Transwell侵袭实验结果显示在IL-8刺激下,敲除ELMO1明显降低MDA-MB-231细胞的侵袭能力,过表达ELMO1明显增强MCF-7细胞的侵袭能力。结论 IL-8能促进MDA-MB-231和MCF-7细胞的趋化运动和侵袭能力,而ELMO1在IL-8诱导的乳腺癌细胞趋化和侵袭作用中发挥重要作用。
Objective To investigate the role of engulfment and cell motility 1 (ELMO1) in the invasion and metastasis of breast cancer cells induced by IL-8. Methods The chemotaxis assay was used to detect the chemotactic ability of breast cancer cells stimulated with different concentrations of IL-8. The expression of ELMO1 in breast cancer cells was detected by Western blot. The expression of ELMO1 in breast cancer cells was transiently transfected by siRNA. MB-231. The overexpression plasmid was used to up-regulate the expression of ELMO1 in breast cancer cell line MCF-7. The chemotaxis and invasion ability of transfected cells were detected by chemotaxis assay and Transwell invasion assay. Results The results of chemotaxis experiments showed that the motility of breast cancer cells MDA-MB-231 and MCF-7 was significantly increased in a dose-dependent manner under the stimulation of IL-8. Western blot results showed that the ELMO1 expression in si ELMO1 / MDA-MB -231 cells in MCF-7 / ELMO1 cells was significantly lower than that in MCF-7 / ELMO1 cells. The results of chemotaxis experiments showed that chemotactic activity of Si ELMO1 / MDA-MB-231 cells stimulated by IL- The ability of chemotaxis in MCF-7 / ELMO1 cells was significantly enhanced. Transwell invasion assay showed that ELMO1 knockdown could significantly reduce the invasiveness of MDA-MB-231 cells under the stimulation of IL-8, Enhance the invasive ability of MCF-7 cells. Conclusion IL-8 can promote the chemotaxis and invasion of MDA-MB-231 and MCF-7 cells, while ELMO1 plays an important role in the chemotaxis and invasion of IL-8 induced breast cancer cells.