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单纯疱疹病毒1型感染相关抑制蛋白(HSV-1 infection related repress protein,HIRRP)是一个结构和功能仍未知的蛋白。我们前期的工作发现HIRRP能在病毒感染的细胞内高表达,表明HIRRP可能在细胞内参与了抗病毒感染这一重要的生物学事件。然而,HIRRP在细胞内参与抗病毒感染的具体作用机理仍不清楚。为此,我们以HIRRP为诱饵构建了pGBKT7-HIRRP载体,用酵母双杂交技术从人肝细胞cDNA文库中筛选出了玻连蛋白(Vitronectin,VTN),并通过体内免疫共沉淀和双荧光共定位实验,以及体外的促细胞增殖实验和病毒入胞干预实验,证实了HIRRP和VTN之间存在着相互作用。初步的功能分析显示,两者的相互作用,具有协同促进细胞抗病毒的效应。由此,我们得出结论,HIRRP和VTN在细胞内能发生相互作用,并猜测这种作用与细胞的抗病毒感染活动相关。
HSV-1 infection-related repressor protein (HIRRP) is a protein whose structure and function remain unknown. Our previous work found that HIRRP can be highly expressed in virus-infected cells, suggesting that HIRRP may be involved in cellular intracellular important biological events such as antiviral infection. However, the specific mechanism by which HIRRP participates in antiviral infection in the cell remains unclear. To this end, we constructed pGBKT7-HIRRP vector using HIRRP as bait. Vitronectin (VTN) was screened from human hepatocyte cDNA library by yeast two-hybrid technique and co-immunoprecipitation and double fluorescence co-localization Experiments, as well as in vitro primed cell proliferation experiments and virus invasion experiments, confirmed the existence of an interaction between HIRRP and VTN. Preliminary functional analysis shows that the interaction between the two has synergistic effects on cell antiviral activity. From this, we conclude that HIRRP and VTN interact within the cell and that this effect is speculated to correlate with cellular anti-viral infection activity.