论文部分内容阅读
目的：探索端粒酶活性与喉癌发生发展的关系。方法：采用重复序列扩增法（TRAP－PCR）检测56例手术切除的喉癌组织和癌旁粘膜组织的端粒酶活性。喉癌组织均经病理证实，喉癌旁粘膜组织中有正常喉粘膜41例，轻度不典型增生15例。结果：喉癌组织端粒酶活性阳性率为91．07％（51／56），正常喉粘膜和轻度不典型增生喉粘膜的阳性率分别为9．76％（4／41）和33．33％（5／15），喉癌组织和癌旁粘膜组织中端粒酶活性阳性率有显著差异（P<0．01）。癌旁上皮端粒酶活性阳性的9例患者其喉癌组织端粒酶活性皆为阳性。结论：端粒酶激活与喉癌的发生发展有密切关系，并可作为喉癌分子诊断的肿瘤标记物。
Objective: To explore the relationship between the telomerase activity and the development of laryngeal carcinoma. Methods: The telomerase activity in 56 cases of resected laryngeal carcinoma tissues and para-cancerous mucosa tissues was detected by TRAP-PCR. Laryngeal cancer tissues were confirmed by pathology, paralytic laryngeal mucosa in the normal laryngeal mucosa in 41 cases, 15 cases of mild dysplasia. Results: The positive rate of telomerase activity in laryngeal carcinoma was 91.07% (51/56). The positive rates of laryngeal mucosa and normal laryngeal mucosa were 9.76% (4/41) and 33, respectively. 33% (5/15), the positive rate of telomerase activity in laryngeal cancer tissues and adjacent mucosa tissues was significantly different (P <0.01). The telomerase activity of laryngeal carcinoma tissues was positive in all 9 patients with para-epithelial telomerase activity. Conclusion: Telomerase activation is closely related to the occurrence and development of laryngeal carcinoma and can be used as a tumor marker for molecular diagnosis of laryngeal cancer.