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目的:探讨钙离子预处理牙本质胶原对原花青素交联效果的影响。方法:牙本质片经磷酸完全脱矿后分为2组,即氯化钙预处理组和未处理组,每组牙本质片分为3个亚组:去离子水组(阴性对照)、戊二醛组(阳性对照)、原花青素组。通过测定牙本质片酶解后的质量损失、羟脯氨酸释放、抗弯强度变化以及透射电镜形貌观察,评估交联后牙本质胶原抗酶解能力和力学性能。结果:戊二醛或原花青素交联处理均可以减少牙本质胶原的降解(P<0.05),并提高其抗弯强度(P<0.05);钙离子预处理可进一步提高原花青素的交联作用,表现为抗酶解能力的提高(P<0.05)和抗弯强度的稳定性(P<0.05)。结论:钙离子预处理脱矿牙本质胶原可增强原花青素对牙本质胶原的交联作用。
Objective: To investigate the effect of pretreatment with calcium ion on the procyanidin cross-linking effect. Methods: Dentin tablets were completely demineralized and divided into two groups: calcium chloride pretreatment group and untreated group. Each dentin tablet was divided into three subgroups: deionized water group (negative control), pentylenetetramine Dialdehyde group (positive control), proanthocyanidin group. The enzymatic hydrolysis resistance and mechanical properties of dentin collagen after cross-linking were evaluated by measuring the mass loss, hydroxyproline release, changes in bending strength and morphological observation of transmission electron microscope. Results: The crosslinking of glutaraldehyde or proanthocyanidins could reduce the degradation of dentin collagen (P <0.05) and increase the flexural strength (P <0.05). Pretreatment with calcium ion could further increase the crosslinking of proanthocyanidins The anti-enzymolysis ability (P <0.05) and the flexural strength stability (P <0.05). Conclusion: Calcium ion pretreatment of demineralized dentin collagen can enhance the proanthocyanidins crosslinking of dentin collagen.