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目的研究常压直接质谱分析技术(AMS)快速鉴别肺癌与癌旁组织的效果及应用。方法在不对肺组织进行任何预处理前提下,采用自制的甲醇萃取辅助的针尖电喷雾离子源对15例肺癌患者的肺癌和癌旁组织样品进行快速质谱分析,萃取剂甲醇的流速为4μL/min,设置离子源为正离子模式,质荷比(m/z)750~850Da,离子传输管温度150℃,喷雾电压3.5 kV,透镜电压65.0 V。应用matlab软件对谱图数据进行分析,肺癌与癌旁组织质谱峰丰度差异采用独立样本t检验;用主成分分析(PCA)方法对肺癌与癌旁组织样品的质谱指纹谱图进行快速分类和鉴别。结果肺癌和癌旁组织样品指纹谱图在m/z为756.7、772.6、780.7、808.7、824.7、832.7等处的相对丰度在肺癌和癌旁组织中差异有统计学意义(P<0.01);PCA分类图中,肺癌组织和癌旁组织样品分别落在不同的区域,两类样品能有效区分。结论 AMS耦合PCA能够有效地将肺癌与癌旁组织样品的质谱指纹区分,从而快速鉴别出肺癌与癌旁组织,并获得相关样品的分子尺度信息,为快速诊断肺癌及肺癌肿瘤标志物研究提供新的思路和策略。
Objective To study the effect and application of rapid differential pressure direct mass spectrometry (AMS) in differentiating lung cancer from paracancerous tissues. Methods Rapid mass spectrometry was performed on lung cancer and para-cancerous tissues in 15 patients with lung cancer using methanol-assisted needle-tip electrospray ionization without any pretreatment of the lung tissue. The methanol extractant flow rate was 4 μL / min The ion source was set as a positive ion mode with a mass-to-charge ratio (m / z) of 750 to 850 Da, an ion transport tube temperature of 150 ° C, a spray voltage of 3.5 kV and a lens voltage of 65.0 V. The matlab software was used to analyze the spectral data. The difference of peak abundance of lung cancer and paracancerous tissues was analyzed by independent sample t-test. The principal component analysis (PCA) method was used to classify the mass spectrum fingerprints of lung cancer and paracancerous tissues Identification. Results The relative abundance of the fingerprints of lung cancer and paracancerous tissues in lung cancer and paracancerous tissues at m / z 756.7, 772.6, 780.7, 808.7, 824.7, 832.7 and so on were statistically significant (P <0.01). PCA classification map, lung cancer and paracancerous tissue samples fell in different regions, two types of samples can be effectively distinguished. Conclusions AMS coupled with PCA can differentiate lung cancer from paracancerous tissue samples effectively, so that lung cancer and paracancerous tissues can be rapidly identified and molecular-scale information of related samples can be obtained, which can provide new information for rapid diagnosis of lung cancer and lung cancer tumor markers Ideas and tactics.