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目的探讨谷胱甘肽耗竭与微囊藻毒素-LR(MCLR)细胞毒性的关系。方法不同剂量的MCLR作用人肝癌细胞HepG2后,采用四甲基偶氮噻唑蓝法检测细胞毒性,并用二硫硝基苯甲酸法测定细胞内还原型谷胱甘肽(GSH)的含量。结果细胞存活率随处理剂量的增加而降低,抗氧化剂N-乙酰半胱氨酸明显减轻MCLR诱导的细胞毒性。细胞内GSH含量显著降低,处理剂量与GSH水平之间呈现剂量-反应和时间-反应关系。结论谷胱甘肽耗竭可能是微囊藻毒素-LR引起细胞毒性的原因之一。
Objective To investigate the relationship between glutathione depletion and cytotoxicity of microcystin-LR (MCLR). Methods After different doses of MCLR were applied to human HepG2 cells, the cytotoxicity was detected by MTT assay and the content of glutathione (GSH) was measured by dithio-nitrobenzoate method. Results The cell viability decreased with the increase of the treated dose, and the antioxidant N-acetylcysteine significantly reduced the MCLR-induced cytotoxicity. The intracellular GSH content was significantly reduced, the dose-response and GSH levels showed dose-response and time-response relationship. Conclusion Glutathione depletion may be one of the causes of microcystin-LR-induced cytotoxicity.