微循环形态学的研究是微循环研究的基础,同时也是解剖学、组织学、病理学、心血管病学的重要课题,对于完整地认识脏器结构,功能及病理改变具有重要意义。用合适的方法显示脏器微血管是进行微血管形态学研究的前提,现有的方法依据原理可分为四类。一、染色法用组织化学或免疫组织化学的方法染色,直接显示微血管,根据显示部位分为管内染色,管壁染色及管周染色。1.管内染色基本原理是利用红细胞中的内源性过氧化物酶及酶标抗体和血浆蛋白结合后的外源性过氧化物酶催化特异底物产生不溶的棕色沉淀显示血管内容物。红细胞中有很强的过氧化物酶活性,在组织切片上滴加联苯胺一过氧化氢反应液,就可以清楚地显示血管内红细胞,但红细 Microcirculation morphological study is the basis of microcirculation research, but also an important issue in anatomy, histology, pathology, cardiovascular disease, for the complete understanding of organ structure, function and pathological changes of great significance. Displaying organ microvessels in appropriate way is the prerequisite for the study of microvascular morphology. The existing methods can be divided into four categories according to the principle. First, the staining method using histochemical or immunohistochemical staining, direct display of microvascular, according to the display site is divided into tube staining, wall staining and tube staining. 1. The basic principle of tube staining is the use of endogenous peroxidase in red blood cells and enzyme-labeled antibodies and plasma protein-binding exogenous peroxidase-catalyzed substrate-specific insoluble brown precipitate showed vascular contents. Erythrocytes have a strong peroxidase activity in the tissue sections dropping benzidine a hydrogen peroxide reaction solution, we can clearly show intravascular red blood cells, but the red fine