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目的:建立测定杜仲中儿茶素含量的高效液相色谱方法。方法:设计正交试验对样品的前处理过程进行优化,采用反相高效液相色谱法测定杜仲中儿茶素含量,色谱柱为Agilent HC-C18(4.6 mm×250 mm,5μm);流动相乙腈-0.4%磷酸溶液(13∶87);流速1.0 mL.min-1,检测波长280 nm。结果:儿茶素在9.620～194.0 mg.L-1线性关系良好(r=0.999 3),平均加样回收率为97.38%,RSD 2.1%。结论:该法简便快速、准确性和重复性好,可用于杜仲中儿茶素的含量测定。
Objective: To establish a HPLC method for the determination of catechin in Eucommia ulmoides. Methods: The orthogonal design was used to optimize the sample pretreatment. The content of catechins in Eucommia ulmoides was determined by reversed-phase high performance liquid chromatography. The mobile phase was Agilent HC-C18 (4.6 mm × 250 mm, 5 μm) Acetonitrile-0.4% phosphoric acid solution (13:87). The flow rate was 1.0 mL.min-1 and the detection wavelength was 280 nm. RESULTS: Catechin showed a good linearity (r = 0.999 3) between 9.620 and 194.0 mg · L -1, with an average recovery of 97.38% and a RSD of 2.1%. Conclusion: The method is simple, rapid, accurate and reproducible, and can be used for the determination of catechin in Eucommia ulmoides.