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合成了新型三脚架结构配体三(2-苯甲酰胺乙基)胺及其稀土Eu3+配合物。通过元素分析、IR、1H-NMR、TG-DTA和摩尔电导对此化合物进行组成和结构推测,结果显示配合物[EuL(NO3)2].NO3是1∶1型配位,3个硝酸根中有两个处于配位内界与Eu3+配位,总配位数为8。通过紫外吸收光谱、荧光发射光谱、粘度法及其与溴化乙啶(EB)的竞争实验研究了配合物与ct-DNA的作用情况,结果显示,配合物与ct-DNA作用时,其紫外吸收产生明显增色效应,荧光强度增强;EB-DNA体系的荧光强度随配合物的加入迅速减弱;配合物的加入使ct-DNA的粘度增加;实验结果证明,配合物与ct-DNA以插入方式结合,其键合常数Kq=8.86×103L·mol-1。凝胶电泳实验表明配合物[EuL(NO3)2].NO3不能将pUC19 DNA选择性的断裂成线型和开环型DNA,并分析讨论了其原因。
Synthesis of a new tripod structure ligand tris (2-benzamidoethyl) amine and rare earth Eu3 + complexes. The composition and structure of the compound were characterized by elemental analysis, IR, 1H-NMR, TG-DTA and molar conductance. The results showed that the complex [EuL (NO3) 2] Two of them are located in coordination with Eu3 + and have a total coordination number of 8. The results of UV-Vis absorption spectra, fluorescence emission spectra, viscometry and their competition with ethidium bromide (EB) show that the interaction between the complexes and ct-DNA shows that the UV- The fluorescence intensity of EB-DNA system decreased rapidly with the addition of the complex. The addition of the complex increased the viscosity of ct-DNA. The experimental results show that the complex with ct-DNA is inserted The bond constant Kq = 8.86 × 103 L · mol -1. Gel electrophoresis showed that the complex [EuL (NO3) 2] .NO3 could not selectively cleave pUC19 DNA into linear and open-loop DNA, and its reason was analyzed and discussed.