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以反相HPLC法分离测定雌三醇(E3)栓剂中E3的含量,采用WatersNora-ParkC18柱,用氯霉素作内标,甲醇-水(50:50)为流动相,方法准确简便。E3的回收率为101.5%,RSD为1.78%。
The content of E3 in the suppository of estriol (E3) was determined by reverse-phase HPLC. The method was accurate and convenient using WatersNora-Park C18 column with chloramphenicol as internal standard and methanol-water (50:50) as mobile phase. The recovery of E3 was 101.5% with a RSD of 1.78%.