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目的 :探讨藤黄酸 (gambogicacid ,GA)诱导体外培养人胃腺癌SGC 790 1细胞凋亡的作用。方法 :通过MTT比色法分析藤黄酸对人胃癌SGC 790 1细胞在 2 4 ,4 8,72h增殖的影响 ,并通过光镜、电镜观察细胞形态学的改变及流式细胞术检测藤黄酸对SGC 790 1肿瘤细胞周期动力学和凋亡诱导率的影响 ,用免疫组化方法检测凋亡相关基因bax和bcl 2的蛋白表达变化。结果 :藤黄酸可明显抑制SGC 790 1细胞的增殖 ,4 8h半数生长抑制剂量 (IC50 )为 1.4 7,1.6 μmol/L的藤黄酸可导致 790 1细胞形态学的改变 ,流式细胞术显示细胞周期亦发生变化 ,G2 /M期细胞增加 ,凋亡率呈时间 剂量相关性。同剂量的藤黄酸还可增加抑癌基因bax和减少诱癌基因bcl 2的蛋白表达量。结论 :藤黄酸可明显抑制SGC 790 1肿瘤细胞的生长 ,诱导细胞凋亡 ,其分子机制可能与其减少Bcl 2 /Bax的比值相关。
Objective: To investigate the effect of gambogic acid (GA) on the apoptosis of human gastric adenocarcinoma SGC 790 1 cells in vitro. METHODS: The effects of gambogic acid on the proliferation of human gastric cancer SGC 790 1 cells at 24, 48, 72 h were analyzed by MTT colorimetry. The changes of cell morphology were observed by light and electron microscopy, and flow cytometry was used to detect the garcinia. The effect of acid on the cell cycle kinetics and apoptosis inducing rate of SGC 790 1 tumor cells was examined by immunohistochemistry to detect the protein expression changes of apoptosis-related genes bax and bcl 2 . RESULTS: Gambogic acid significantly inhibited the proliferation of SGC 790 1 cells. Garcinic acid with an IC50 of 1.4 7, 1.6 μmol/L at 48 h could cause morphological changes in 790 1 cells. Flow cytometry It also showed that the cell cycle also changed, G2 / M phase cells increased, the rate of apoptosis was time-dose correlation. The same dose of gambogic acid can also increase the tumor suppressor gene bax and decrease the protein expression of the oncogene bcl 2 . Conclusion : Gambogic acid can obviously inhibit the growth of SGC 790 1 tumor cells and induce apoptosis. The molecular mechanism may be related to the decrease of Bcl 2 /Bax ratio.