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本试验通过采用不同浓度的NaCl和Na2CO3胁迫萌发期的西兰花(Brassica oleracea L.var.botrytis L.)种子,调查了其发芽和胚保护酶活性等指标。研究结果表明,低浓度(≤20 mmol/L)的NaCl胁迫对西兰花各发芽指标有促进作用,在等Na+浓度胁迫下,各Na2CO3胁迫对各发芽指标均表现出抑制作用。Na2CO3胁迫处理胚的SOD和POD活性低于NaCl胁迫处理,MDA含量高于NaCl胁迫处理。综合分析表明,西兰花耐盐阈值和耐碱阈值分别为445.5 mmol/L和64.1 mmol/L。
In this experiment, the seeds of Brassica oleracea L.var.botrytis L. were germinated under different NaCl and Na2CO3 stress, and their germination and embryo protective enzyme activities were investigated. The results showed that NaCl stress at low concentration (≤20 mmol / L) promoted the germination of broccoli, and under the stress of Na +, each Na2CO3 stress inhibited the germination of each broccoli. The activity of SOD and POD of embryos treated with Na2CO3 was lower than that of NaCl, and the content of MDA was higher than that of NaCl treated. Comprehensive analysis showed that salt tolerance threshold and alkali tolerance threshold of broccoli were 445.5 mmol / L and 64.1 mmol / L, respectively.