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目的对2011年7月广州市发生的一起细菌性食物中毒进行病原学特征分析。方法对送检病人粪便、厨工肛拭及食物等样品进行荧光PCR检测和分离培养,对分离株进行生化鉴定,血清分型、脉冲场凝胶电泳(PFGE)分型和药敏分析。结果从病人粪便、肛拭和厨房抹布共分离到18株利齐菲尔德沙门菌,为相同的PFGE型别,药敏结果显示菌株对实检和定阿米卡星,庆大霉素及妥布霉素耐药,对头孢噻肟、亚胺培南、甲氧苄啶/磺胺甲恶唑等15种抗生素均敏感。结论实验判定这是一起由同一克隆型的利齐菲尔德沙门菌污染厨房环境所引起的食物中毒事件,PFGE分型法为暴发溯源提供分子流行病学证据和支持。
Objective To analyze the etiological characteristics of a bacterial food poisoning occurred in Guangzhou City in July 2011. Methods The samples of feces, kitchen workers, swabs and food samples were detected by fluorescence PCR. The isolates were biochemically identified, serotyped, pulsed field gel electrophoresis (PFGE) typing and drug susceptibility analysis. Results Eighteen strains of Salmonella Lichtenstein were isolated from the patient’s feces, anal swabs and kitchen rags. The same type of PFGE was observed. The drug susceptibility results showed that the isolates of the strains were resistant to the tests and amikacin, gentamycin and gemcitabine Mycotoxin resistance, to cefotaxime, imipenem, trimethoprim / sulfamethoxazole and other 15 kinds of antibiotics are sensitive. Conclusion It is experimentally determined that this is a food poisoning event caused by the contamination of the kitchen environment caused by the same cloned Lichtenstein spp. PFGE typing provides molecular epidemiological evidence and support for the origin of the outbreak.