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本文建立了在常温常速离心条件下进行样品预处理的人血浆三尖杉酯碱高效液相色谱荧光测定法。本法批内CV%为3.08～3.47%,批间CV%为4.36～5.28%,回收率范围为93.6～95%,标准曲线在12.5～500ng/ml范围内线性良好(r=0.998)。方法简单、快速、准确,适于临床应用。
In this paper, a set of human plasma homoharringtonine high performance liquid chromatography fluorescence detection method was developed for sample pretreatment under normal temperature and normal speed centrifugation conditions. The CV% was 3.08 ~ 3.47%. The CV% was 4.36 ~ 5.28%. The recovery range was 93.6 ~ 95%. The calibration curve was linear over the range of 12.5 ~ 500ng / ml (r = 0.998). The method is simple, rapid and accurate, suitable for clinical application.