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目的分析南通地区沙门菌的耐药性,初步阐明定位于质粒上的整合子介导的耐药性传播的分子机制。方法收集2008—2011年南通地区食品中分离的沙门菌67株,采用K-B纸片扩散法进行药敏试验,确定其耐药谱;筛选出多重耐药性菌株,并以大肠杆菌为受体菌进行接合试验。PCR分析受体菌整合子特异序列,并进行分型检测。结果南通地区检测出的沙门菌中有74.6%的沙门菌株(50/67)对抗生素产生耐药性,其中31.3%(21/67)对超过1种抗生素有耐药性,发展成为多重耐药性沙门菌;沙门菌Ⅰ类整合子检出率为20.9%(14/67)。结合实验还发现沙门菌Ⅰ类整合子可以介导耐药性向大肠杆菌的传播。结论南通地区食品分离的沙门菌Ⅰ类整合子检出率较高。沙门菌携带的Ⅰ类整合子是引起耐药性在种间和种内传播的重要因素。
Objective To analyze the drug resistance of Salmonella in Nantong and elucidate the molecular mechanism of integron-mediated drug resistance transmission. Methods Sixty-seven strains of Salmonella isolated from food in Nantong from 2008 to 2011 were collected and susceptible to antibiotics were tested by KB disk diffusion method. Multi-resistant strains were screened and Escherichia coli Perform the bonding test. PCR specific receptor integrin integration sequence, and the typing test. Results 74.6% of Salmonella strains (50/67) in Salmonella detected in Nantong area were resistant to antibiotics, of which 31.3% (21/67) were resistant to more than one antibiotic and developed into multi-drug resistance Salmonella; Salmonella class I integron detection rate was 20.9% (14/67). Combined with experiments also found that Salmonella class Ⅰ integron can mediate the spread of drug-resistant E. coli. Conclusion The detection rate of Salmonella type Ⅰ integron isolated from food in Nantong area is high. The class I integron carried by Salmonella is an important factor that causes drug resistance to spread in and between species.