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目的探讨苏州地区妇女宫颈感染人乳头状瘤病毒(HPV)的基因型分布及其临床意义。方法采用基因扩增结合基因芯片技术对4 862例宫颈上皮细胞标本进行23种HPV基因分型检测。结果宫颈上皮细胞HPV阳性率为31.74%(1543/4 862)。其中,一重感染、多重感染的阳性率分别为21.43%(1 042/4 862)、10.30%(501/4 862)。前7位高危型HPV分别为16、58、52、33、18、56、31型,占59.09%,而低危型HPV分别为11、6、43型,占23.70%。结论基因扩增结合基因芯片技术可应用于宫颈细胞HPV分型检测。
Objective To investigate the genotypic distribution and clinical significance of cervical intraepithelial infection of human papillomavirus (HPV) in Suzhou area. Methods Twenty-three HPV genotypes were detected in 4 862 cervical epithelial samples by gene amplification combined with gene chip technique. Results The positive rate of HPV in cervical epithelial cells was 31.74% (1543/4 862). Among them, the positive rates of severe infections and multiple infections were 21.43% (1042/4 862) and 10.30% (501/4 862), respectively. The first seven high-risk HPV types were 16,58,52,33,18,56,31, accounting for 59.09%, while the low-risk HPV types were 11,6,43, accounting for 23.70%. Conclusion Gene amplification combined with gene chip technology can be applied to detect HPV types of cervical cells.