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目的:探讨外周血白蛋白(Alb)基因在肝癌早期诊断和鉴别诊断中的临床价值。方法:以巢式聚合酶链反应(RT-PCR)扩增外周血中Alb的基因片段分析在原发性肝癌(HCC)、慢性肝病和非肝病患者外周血中血白蛋白基因表达。结果:67例肝癌患者外周血中Alb基因的检出率为59.7%,明显高于肝硬化、慢性肝炎和肝外肿瘤组患者(P<0.01),但与急性肝炎组无明显差别(P>0.05);在Ⅰ、Ⅱ和Ⅲ期肝癌中,Alb基因片段阳性率分别为33.3%、36.8%和76.9%;肝内有无转移的肝癌组间存在明显差异(P<0.05),尤其在伴有肝外远处转移的肝癌中,Alb基因全数阳性(100.0%)。结论:分析外周血中Alb基因有助于肝癌的诊断、肝癌远处转移判别的监测。
Objective: To investigate the clinical value of Alb gene in the early diagnosis and differential diagnosis of liver cancer. Methods: The gene fragment of Alb in peripheral blood was amplified by nested polymerase chain reaction (RT-PCR) and the expression of albumin gene in peripheral blood of patients with primary liver cancer (HCC), chronic liver disease and non-liver disease was analyzed. Results: The detection rate of Alb gene in peripheral blood of 67 patients with hepatocellular carcinoma was 59.7%, which was significantly higher than that of patients with cirrhosis, chronic hepatitis and extrahepatic tumor (P <0.01), but no significant difference with acute hepatitis (P> 0.05). The positive rates of Alb gene in stage Ⅰ, Ⅱ and Ⅲ were 33.3%, 36.8% and 76.9%, respectively. There was a significant difference (P <0.05) between the groups with or without metastasis in liver All of the Alb genes were positive (100.0%) in HCC with distant extrahepatic metastases. Conclusion: The analysis of Alb gene in peripheral blood is helpful for the diagnosis of liver cancer and distant metastasis of liver cancer.