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目的:研究补肺丸中黄芪甲苷的含量测定方法。方法:采用高效液相色谱-蒸发光散射(HPLC-ELSD)法,以Wa-ters SymmetryShieldTM C18色谱柱分析;流动相乙腈-水(36∶64);流速1.0 mL.min-1;漂移管温度90℃;氮气压力25 psi。结果:黄芪甲苷在0.367 2～2.203 2μg呈良好的线性关系,r=0.999 8;平均回收率为95.49%,RSD为2.5%(n=6)。结论:该方法简便、快捷、灵敏、准确,可作为补肺丸的质量控制方法。
Objective: To study the method of determination of astragaloside IV in Bufei pills. METHODS: HPLC-ELSD was performed on a Wa-ters Symmetry Shielded C18 column. The mobile phase was acetonitrile-water (36:64), the flow rate was 1.0 mL.min-1. The temperature of the drift tube 90 ° C; nitrogen pressure 25 psi. Results: Astragaloside showed a good linear relationship between 0.367 2 and 2.203 2 μg (r = 0.999 8). The average recovery was 95.49% and the RSD was 2.5% (n = 6). Conclusion: The method is simple, rapid, sensitive and accurate and can be used as a quality control method for Bufei Pills.