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目的 :探讨聚焦超声治疗小鼠宫颈上皮内瘤样变(cervical intraepithelial neoplasia,CIN)的疗效及其可能的作用机制。方法 :利用二甲基苯蒽原位诱发小鼠CINⅠ动物模型,随机分为治疗组(20只)和对照组(20只)。治疗组给予聚焦超声治疗;对照组采用与治疗组相同的操作方法进行治疗,但仪器无功率输出。治疗后2周,应用HE染色法和免疫组织化学法分别检测小鼠宫颈组织的病理学变化以及小鼠宫颈组织中p16、Ki67、Fas和Fas L的表达。结果 :成功建立了小鼠CINⅠ动物模型。治疗组中有15只小鼠治疗后恢复为正常宫颈组织,4只小鼠治疗后仍为CINⅠ,死亡1只,治疗有效率为75%(15/20);对照组中有11只小鼠治疗后仍为CINⅠ,6只发展为CINⅡ,1只发展为CINⅢ,死亡2只,治疗有效率为0%;2组间差异有统计学意义(P<0.01)。治疗组小鼠宫颈组织中p16、Ki67和Fas L的强阳性表达率低于对照组,而Fas的强阳性表达率高于对照组(P值均<0.01)。结论 :聚焦超声可有效治疗小鼠CINⅠ,这可能与宫颈组织中p16、Ki67、Fas和Fas L表达水平的变化有关。
Objective: To investigate the therapeutic effect of focused ultrasound on cervical intraepithelial neoplasia (CIN) and its possible mechanism. Methods: CIN I mice were induced by dimethylbenzyl anthracene in situ and were randomly divided into treatment group (n = 20) and control group (n = 20). The treatment group was given focused ultrasound; the control group was treated with the same procedure as the treatment group, but the instrument had no power output. Two weeks after treatment, the pathological changes of mouse cervical tissue and the expression of p16, Ki67, Fas and Fas L were detected by HE staining and immunohistochemical method respectively. Results: Mouse CIN I animal model was successfully established. In the treatment group, 15 mice recovered to normal cervical tissue after treatment, 4 mice remained CINⅠ after treatment, and 1 died. The effective rate was 75% (15/20). In the control group, 11 mice After treatment, CINⅠ still remained, 6 CINⅡ, CIN Ⅲ 1, and 2 deaths. The effective rate was 0%. The difference between the two groups was statistically significant (P <0.01). The strong positive expression rate of p16, Ki67 and Fas L in cervical tissue of mice in treatment group was lower than that in control group, while the strong positive expression rate of Fas was higher than that in control group (P <0.01). Conclusion: Focused ultrasound can effectively treat mouse CIN Ⅰ, which may be related to the changes of p16, Ki67, Fas and Fas L in cervical tissue.