目的研究氯仿对大鼠皮肤屏障功能的影响。方法对３天龄ＳＤ大鼠的活体皮肤进行氯仿涂搽、接触１０和２０ｍｉｎ的处理，采用静式渗透模型，以氚水经皮渗透量作为皮肤屏障功能的评价指标。结果３个氯仿接触组的氚水经皮渗透量均随时间递增，高于对照组，并在一定时点具有统计学意义（Ｐ＜０．０５），且３个氯仿接触组的氚水经皮渗透量的大小顺序为氯仿接触２０ｍｉｎ＞接触１０ｍｉｎ＞涂搽。结论经氯仿处理后皮肤的屏障功能降低，与皮肤接触氯仿的水平相关。 Objective To study the effect of chloroform on the skin barrier function in rats. Methods The skin of three-day-old SD rats were treated with chloroform, 10 and 20 min exposure. The osmotic permeability of tritiated water was used as the evaluation index of skin barrier function. Results The transdermal permeation of tritiated water in all three chloroform-exposed groups increased with time and was higher than that of the control group (P <0.05), and the tritiated water of the three chloroform-exposed groups Skin penetration in the order of the size of chloroform contact 20min> contact 10min> Tu paint. Conclusion The barrier function of the skin decreased after treated with chloroform, which is related to the level of skin contact with chloroform.